abstract：:A direct assay for plasminogen activator (PA) was developed. It employed polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and beta-mercaptoethanol to monitor PA-mediated conversion of single chain, 125I-plasminogen to two chain plasmin. By incorporating Triton X-100, albumin and trasylol in...

journal_title：Thrombosis research

authors： Mussoni L,Lawrence D,Loskutoff DJ

更新日期：1984-05-01 00:00:00

abstract：:Upon incubation for 24 hours with [3H]arachidonic acid (AA, 1 mu Ci/ml), cultured endothelial cells from human umbilical vein incorporated one half of the added radioactivity, mostly into phospholipids (83% of the total cell radioactivity). Distribution of the label between the various phospholipid classes was found t...

journal_title：Thrombosis research

authors： Thomas JM,Hullin F,Chap H,Douste-Blazy L

更新日期：1984-04-15 00:00:00

abstract：:Platelets from rats fed a diet high in linoleic acid (6%) bound increased amounts of fibrinogen on stimulation with ADP, compared to those from rats fed diets with low (2%) or no linoleic acid. However, this increased fibrinogen binding was associated with a decrease in platelet aggregation induced by ADP. Changes in ...

journal_title：Thrombosis research

authors： McGregor L,Toor B,McGregor JL,Renaud S,Clemetson KJ

更新日期：1984-04-01 00:00:00

abstract：:We purified the urinary procoagulant from frozen human urine by introducing phenyl-Sepharose hydrophobic chromatography. By this method, the apoprotein of the procoagulant and the lipid-like substance were separately recovered. Upon reassociation with the lipid-like substance or exogenous crude phospholipids, the apop...

journal_title：Thrombosis research

authors： Kurosawa S,Matsuda M,Aoki N

更新日期：1984-03-15 00:00:00

abstract：:Cooperative effects of PAF-acether and arachidonic acid in blood platelet activation were studied in human platelet-rich plasma. Using a combination of low concentrations of PAF-acether and subthreshold amounts of exogenous arachidonic acid an enhancement of aggregation and an increased formation of malondialdehyde ar...

journal_title：Thrombosis research

authors： Ostermann G,Till U,Thielmann K

更新日期：1984-02-15 00:00:00

abstract：:Amino sugars and basic amino acids inhibit platelet aggregation and the activity of the endogenous platelet lectin, yet, relatively high concentrations (approximately 30 mM) are required for the inhibition. If cooperative interactions are involved in these platelet surface activities, oligomers of primary amines shoul...

journal_title：Thrombosis research

authors： Agam G,Gartner TK,Livne A

更新日期：1984-02-01 00:00:00

abstract：:Plasma from patients with thrombotic thrombocytopenic purpura (TTP) caused the aggregation of washed human platelets, which was inhibited by preincubation with normal plasma. Using salt fractionation, ion exchange chromatography, and preparative agarose gel electrophoresis, we purified a protein from normal plasma whi...

journal_title：Thrombosis research

authors： Lian EC,Mui PT,Siddiqui FA,Chiu LL

更新日期：1984-01-01 00:00:00

abstract：:The effects of locally deposited fibrin and of tranexamic acid-induced antifibrinolysis on forming granulation tissue were studied in the light of a recently developed method for treatment of postoperative fistulas by occlusion with a fibrin clot. Perforated teflon cylinders, either empty or fibrin-filled, were implan...

journal_title：Thrombosis research

authors： Hedelin H,Teger-Nilsson AC,Peterson HI,Pettersson S

更新日期：1984-01-01 00:00:00

abstract：:Ethanol (88-880 mmol/l) inhibited the formation of proaggregatory, vasoconstricting thromboxane A2 (TxA2) during whole blood clotting and during thrombin-induced aggregation of platelet rich plasma. This inhibition was counteracted by the addition of exogenous arachidonic acid, which suggested that ethanol suppressed ...

journal_title：Thrombosis research

authors： Toivanen J,Ylikorkala O,Viinikka L

更新日期：1984-01-01 00:00:00

abstract：:The concentration of the glycoprotein (GP) IIb-IIIa complex in thrombasthenic platelets of 8 patients of 6 families has been estimated. In the thrombasthenic platelets of 3 patients this complex is absent (thrombasthenia type I and subtype I). In 2 patients only traces are detectable and in 3 patients GP IIb-IIIa comp...

journal_title：Thrombosis research

authors： Herrmann FH,Meyer M,Gogstad GO,Solum NO

更新日期：1983-12-15 00:00:00

abstract：:The interaction of platelets with collagen involves short aminoacid sequences which recur along the fibres. Platelet aggregation by collagen and serotonin release is inhibited by a synthetic octapeptide LYS-PRO-GLY-GLU- PRO-GLY-PRO-LYS- derived from type III collagen. In contrast, this octapeptide inhibits only weakly...

journal_title：Thrombosis research

authors： Karniguian A,Legrand YJ,Lefrancier P,Caen JP

更新日期：1983-12-15 00:00:00

abstract：:Effects of Ca2+ on phospholipid methylation in rabbit platelet membranes were studied using S-adenosyl-L-[3H-methyl]methionine as a substrate. The methylation was inhibited to 30% of the basal activity with 100 microM Ca2+. The inhibition was completely counteracted by various calmodulin antagonists. Their concentrati...

journal_title：Thrombosis research

authors： Taniguchi S,Mori K,Hayashi H,Fujiwara M,Fujiwara M

更新日期：1983-12-01 00:00:00

abstract：:Two types of LMW heparin were prepared by gel filtration of standard heparin (LMW fraction) and by degradation of heparin by nitrous acid (LMW fragment), respectively. The effects on factor Xa inhibition (XaI), APTT, platelet aggregation and AT III level of these preparations were studied after subcutaneous administra...

journal_title：Thrombosis research

authors： Bergqvist D,Hedner U,Sjörin E,Holmer E

更新日期：1983-11-15 00:00:00

abstract：:In a double-blind, placebo-controlled trial of 40 patients requiring aortocoronary vene transplant surgery, prostacyclin (PGI2) was infused in a dose of 8 ng/kg/min throughout cardiopulmonary bypass. When compared with the placebo-group, the patients treated with PGI2 were found to have significantly higher platelet c...

journal_title：Thrombosis research

authors： Heinrich D,Schleussner E,Wagner WL,Sellmann-Richter R,Hehrlein FW

更新日期：1983-11-15 00:00:00

abstract：:Aggregation responses of platelet-rich plasma (PRP) from monocrotaline pyrrole (MCTP)-treated rats were examined to help elucidate the role of platelets in MCTP-induced pulmonary hypertension. PRP from rats treated one or four days earlier with MCTP (5 mg/kg, i.v.) or vehicle exhibited the same slope and maximum aggre...

journal_title：Thrombosis research

authors： Hilliker KS,Deyo JA,Bell TG,Roth RA

更新日期：1983-11-01 00:00:00

abstract：:Isolated rat liver macrophages (Kupffer cells) produce a factor(s) which stimulates fibrinogen synthesis 5-8 fold in hepatocyte cultures. The production of the stimulatory factor reached maximum during the first 24 hours the cells were in culture. When the cell culture medium was chromatographed on a calibrated Sephad...

journal_title：Thrombosis research

authors： Sanders KD,Fuller GM

更新日期：1983-10-15 00:00:00

abstract：:Human gel-filtered platelets or platelet-rich plasma were stimulated by sodium arachidonate or by ADP in the presence of two compounds known to inhibit ADP mediated aggregation and secretion - ATP and N-ethylmaleimide. Using gel-filtered platelets and the lowest concentration of agonist necessary to elicit maximum agg...

journal_title：Thrombosis research

authors： Burch JW,Lamb RJ

更新日期：1983-09-01 00:00:00

abstract：:This study compares selected properties of purified tryptic fragments of human von Willebrand (vW) protein in order to better understand how the M(r) 116,000 fragment retains activity in ristocetin-induced platelet agglutination. Wheat germ agglutinin (WGA) aggregation of platelet-rich plasma was potentiated by preinc...

journal_title：Thrombosis research

authors： Martin SE,Francis CW,Marder VJ

更新日期：1983-08-01 00:00:00

abstract：:Disadvantages of current bleeding time methods include poor reproducibility, limited repeatability, and at times scar formation. We report a new, in vitro technique which circumvents these problems. Venous blood is collected in sodium citrate, stored capped in a siliconized tube at 37 degrees C until use, and made to ...

journal_title：Thrombosis research

authors： Uchiyama S,Stropp JQ,Claypool DA,Didisheim P,Dewanjee MK

更新日期：1983-07-01 00:00:00

abstract：:We examined the effects of verapamil on platelet function. Verapamil (0.5 micrograms/ml) in this study inhibited platelet aggregation induced by threshold amounts of ADP, AA, and epinephrine. With higher concentrations of aggregating stimuli, verapamil caused a dose-dependent inhibition of aggregation. Verapamil reduc...

journal_title：Thrombosis research

authors： Mehta J,Mehta P,Ostrowski N,Crews F

更新日期：1983-06-01 00:00:00

abstract：:Platelet aggregation, lipoprotein lipase activity, coagulation parameters and routine blood chemistry were measured in a randomised study of 21 surgical patients before, immediately after and 3 months after operation. Sodium heparin 5000 IU was given subcutaneously to 11 patients every 12 hours for 7 days, the first i...

journal_title：Thrombosis research

authors： Törngren S,Norén I,Forsskåhl B,Sipilä H

更新日期：1983-06-01 00:00:00

abstract：:The antithrombotic effect of aspirin (ASA) and dipyridamole (DIP) was evaluated in rabbits in which platelet thromboxane A2 (TXA2) and arterial prostacyclin (PGI2) were measured. An intracarotid cannula thrombosis model previously shown to be sensitive to antiplatelet agents was used. Prostaglandins were determined by...

journal_title：Thrombosis research

authors： Louie S,Gurewich V

更新日期：1983-05-15 00:00:00

abstract：:Several inflammatory particles (zymosan, two forms of asbestos, carrageenan) and the soluble inflammatory agent dextran sulphate induced a dose- and time-dependent increase in thromboplastin activity of cultured human monocytes. Two weakly inflammatory particles (latex and anatase) had little effect. The increase larg...

journal_title：Thrombosis research

authors： Dean RT,Prydz H

更新日期：1983-05-15 00:00:00

abstract：:Change in the density of circulating platelets in monkeys was studied by using a method that removed greater than 90% of the platelet population from whole blood. These were labelled with 51-Cr and reinjected, having been protected by prostacyclin while in vitro. Platelets were separated into density subpopulations th...

journal_title：Thrombosis research

authors： Martin JF,Penington DG

更新日期：1983-04-15 00:00:00

abstract：:A nephelometric method is described for determination of plasminogen and two types of plasmin inhibitors in human plasma having different affinity toward plasmin. This method is based on the kinetic analysis of effects of whole plasma and plasmin inhibitor fraction obtained from plasma on the activity of exogenously a...

journal_title：Thrombosis research

authors： Saito Y,Takabayashi K,Tsukada T,Inada Y

更新日期：1983-04-01 00:00:00

abstract：:Homogenous preparations of human and rabbit platelets synthesized platelet-activating factor (PAF) when stimulated by calcium ionophore A23187. PAF was isolated by high performance liquid chromatography (HPLC) on a Lichrosorb Si60 column. PAF was well separated from other phospholipids especially sphingomyelin and lys...

journal_title：Thrombosis research

authors： Alam I,Smith JB,Silver MJ

更新日期：1983-04-01 00:00:00

abstract：:An extensive study of the pharmacokinetics and the pharmacokinetics of the profibrinolytic activity of Defibrotide (D) in the rabbit has been made. a two compartment model was used to describe D's pharmacokinetics. The parameters of drug disposition and the rate constant of release from tissues to central compartment ...

journal_title：Thrombosis research

authors： Pescador R,Mantovani M,Prino G,Madonna M

更新日期：1983-04-01 00:00:00

abstract：:Experimental disseminated intravascular coagulation (DIC) can be induced by 4-h sustained infusion of endotoxin in a dose of 100 mg/kg in rats. The experimental model of DIC in rats was used to study the preventive effect of dipyridamole against DIC. Before the infusion of endotoxin, 0.5, 5.0 or 50.0 mg/kg of dipyrida...

journal_title：Thrombosis research

authors： Yoshikawa T,Murakami M,Furukawa Y,Takemura S,Kondo M

更新日期：1983-03-15 00:00:00

abstract：:The effect of warfarin-induced factor VII deficiency on the skin bleeding time was investigated in 10 consecutive patients with mean prothrombin times of 33 sec and factor VII levels of 7%. Bleeding times in all but one subject were entirely within the normal range. We conclude that warfarin administration, even to th...

journal_title：Thrombosis research

authors： Green D,Ts'ao CH

更新日期：1983-02-15 00:00:00

abstract：:Evidence is presented for in vitro high affinity binding of serotonin (5-HT) by beta-thromboglobulin (beta TG) and platelet factor 4 (PF4) from human blood. Results include: 1) identification by radioimmunoassay of PF4 in specifically bound material obtained by 5-HT affinity chromatography of human platelet extracts; ...

journal_title：Thrombosis research

authors： Heemstra VL

更新日期：1983-02-01 00:00:00

abstract：:The perfusion technique to measure the relative adhesive and thrombogenic indices of vascular prostheses by a quantitative scanning electron microscopy has been described. To normalize the experiments with different samples of plasma we compared the adhesive and thrombogenic properties of the analyzed prostheses with ...

journal_title：Thrombosis research

authors： Dolgov VV,Zaikina OE,Repin VS,Smirnov VN,Bujanovsky VL,Kazanchyan PO,Pokrovsky VA

更新日期：1983-02-01 00:00:00

abstract：:Porcine and human umbilical vein and adult blood vessels were studied for the presence and synthesis of factor VIII related antigen (VIIIR:Ag) and fibronectin (Fn) by immunofluorescence histology and immunoautoradiography. Investigation of human tissue confirmed the widespread distribution of VIIIR:Ag on the endotheli...

journal_title：Thrombosis research

authors： Giddings JC,Jarvis AL,Bloom AL

更新日期：1983-02-01 00:00:00

abstract：:Clot adherence to peritoneal cavity mesothelium was studied in rats in an attempt to contribute to the problem of thrombus embolization. The adherence was decreased by platelets and the active constituent was identified as adenosine diphosphate which decreased the sticking of fibrin to cellular linings as well as to a...

journal_title：Thrombosis research

authors： Hladovec J

更新日期：1982-12-15 00:00:00

abstract：:In rabbits, a clear microscopic image of individual, fluorescent blood platelets flowing in a mesenteric microvessel could be obtained over the whole cross-sectional area of the vessel, following an intravenous injection of a solution containing 5-15 mg of the fluorochrome Acridine Red. Most of the circulating platele...

journal_title：Thrombosis research

authors： Tangelder GJ,Slaaf DW,Reneman RS

更新日期：1982-12-15 00:00:00

abstract：:The specific thromboxane A2/prostaglandin H2 (TXA2/PGH2) antagonist 13-azaprostanoic acid (13-APA) reverses platelet aggregation stimulated by TXA2/PGH2 and the prostaglandin endoperoxide analog U46619. The present report demonstrates that the deaggregatory properties of 13-APA are potentiated by prostacyclin (PGI2). ...

journal_title：Thrombosis research

authors： Parise LV,Venton DL,Le Breton GC

更新日期：1982-12-15 00:00:00

abstract：:Human and bovine alpha-thrombins (greater than 90% alpha form) with high fibrinogen clotting activities (approximately 3,000 U.S. units/mg protein) exhibit similar Michaelis menten kinetics with the p-nitroanilide tripeptide substrates Tos-Gly-Pro-arg-pNA (Chromozym-TH) and D-Phe-Pip-Arg-pNA (S-2238). The kinetic para...

journal_title：Thrombosis research

authors： Lottenberg R,Hall JA,Fenton JW 2nd,Jackson CM

更新日期：1982-11-01 00:00:00

abstract：:A new method, based on the observation that leukocytes contain PGI2-synthetase activity, was used to measure the effect of pharmacological levels of sulphinpyrazone on the TXA2 and PGI2 production in whole blood. Four human volunteers took 400 mg sulphinpyrazone twice daily for 5 1/2 days. Blood was drawn before the s...

journal_title：Thrombosis research

authors： Badenhorst PN,Deckmyn H,Vermylen J

更新日期：1982-10-01 00:00:00

abstract：:This study evaluates the role of neutrophil granulocytes in mediating acute phase stimulation of fibrinogen synthesis. Turpentine was administered to neutropenic and non-neutropenic rats and fibrinogen synthetic rates measured in an isolated liver perfusion system. Using the (14C) carbonate technique for the measureme...

journal_title：Thrombosis research

authors： Kernoff L,Colman J

更新日期：1982-10-01 00:00:00

abstract：:The soluble fibrin monomer complexes (SFMC) in 154 obstetric patients with possible disseminated intravascular coagulation (DIC) were evaluated in SDS polyacrylamide gel electrophoresis (PAGE) after precipitation with B-alanine. Other coagulation tests were performed on these patients. The patients were classified int...

journal_title：Thrombosis research

authors： Aznar J,Gilabert J,Estelles A,Fernandez MA,Villa P,Aznar JA

更新日期：1982-09-15 00:00:00

abstract：:A significantly higher platelet malondialdehyde-like material (MDA--LM) content after physical and N-ethylmaleimide (NEM) stimulation is found in chronic ischemic heart disease (CHD) patients when compared to the control group. In subjects under aspirin treatment "in vivo" (1 gr/day) no difference is found between CHD...

journal_title：Thrombosis research

authors： Valles J,Aznar J,Santos MT,Fernandez MA

更新日期：1982-09-01 00:00:00